Figure 4.

Normalized pair–distance and dimensionless Kratky plots for PLCε variants alone and in complex with Rap1A^G12V.A, the normalized P(r) functions for PH-COOH and Rap1A^G12V–PH-COOH are similar, with D_max values of ∼162 and ∼165 Å, respectively. B, comparison of PH-COOH (black circles) and Rap1A^G12V–PH-COOH (gray squares) shows that the complex is more compact and globular than PH-COOH alone, as evidenced by the more bell-shaped curve and convergence at lower qR_g. C, the normalized P(r) functions for EF3-COOH and Rap1A^G12V–EF3-COOH reveal that binding of Rap1A^G12V induces substantial conformational changes that lead to a more compact structure. This is further supported by the ∼30 Å decrease in D_max for the Rap1A^G12V–EF3-COOH complex. D, comparison of EF3-COOH (blue circles) and Rap1A^G12V–EF3-COOH (light blue squares). Rap1A^G12V binding induces conformational changes that result in a more compact and globular solution structure.