Table 1.
Stability, basal activity, and fold-activation by Rap1A of PLCε variants
The data represent at least two independent experiments performed in duplicate ± S.D. The results are based on one-way analysis of variance followed by Dunnett's multiple comparisons test versus PLCε PH-COOH.
| PLCε variant | Tm | Basal specific activitya | Fold activation by Rap1AG12V (n) |
|---|---|---|---|
| ° C (n) | nmol IP3/min/nmol PLCε variant) (n) | ||
| PH-COOHb | 51.3 ± 0.72 (6) | 360 ± 120 (9) | 3.0 (4) |
| EF3-COOH | 51.7 ± 0.12 (4) | 450 ± 50 (3) | N/A (3) |
| PH-C2b | 48.3 ± 1.00 (4)c | 80 ± 20 (5)c | N/A (2) |
| PH-COOH K2150A | 50.5 ± 0.86 (3) | 160 ± 30 (4)d | 1.8 (2) |
| PH-COOH K2152A | 49.6 ± 0.79 (3) | 240 ± 20 (3) | 0.9 (3) |
| PH-COOH Y2155A | 49.8 ± 1.06 (3) | 250 ± 40 (5) | 1.4 (3) |
| PH-COOH L2158A | 51.2 ± 0.39 (3) | 270 ± 100 (3) | 1.0 (3) |
| PH-COOH L2192A | 51.5 ± 0.71 (3) | 270 ± 50 (3) | 1.3 (3) |
| PH-COOH F2198A | 51.5 ± 0.45 (3) | 280 ± 100 (3) | 1.2 (3) |
a
Maximum specific activities were measured at a single time point.
b
Tm and specific activity for these variants were previously reported and are included here for comparison (24).
c
p ≤ 0.0002.
d
p ≤ 0.0028.