Table 2.
PH-COOHa | Rap1AG12V–PH-COOH | EF3-COOH | Rap1AG12V–EF3-COOH | |
---|---|---|---|---|
Guinier analysis | ||||
I(0) (Arb.) | 64.92 ± 0.21 | 1.66 ± 0.003 | 78.10 ± 0.43 | 26.96 ± 0.50 |
Rg (Å) | 42.7 ± 0.17 | 42.4 ± 0.12 | 41.9 ± 0.43 | 36.8 ± 0.50 |
q min (Å−1) | 0.0174 | 0.0043 | 0.0084 | 0.0094 |
q range (Å−1) | 0.0174–0.0314 | 0.0043–0.0306 | 0.0084–0.0309 | 0.0094–0.352 |
P(r) analysis | ||||
I(0) (Arb.) | 65.96 ± 0.21 | 1.67 ± 0.03 | 79.43 ± 0.63 | 27.81 ± 0.21 |
Rg (Å) | 44.79 ± 0.19 | 43.70 ± 0.16 | 44.48 ± 0.61 | 39.47 ± 0.43 |
Dmax (Å) | 162 | 165 | 175 | 145 |
Porod volume (Å−3) | 191,000 | 237,000 | 179,000 | 178,000 |
q range (Å−1) | 0.0174–0.308 | 0.0042–0.350 | 0.0087–0.387 | 0.0052–0.366 |
The data for the PLCε PH-COOH variant was previously published, and is included for comparison (24).