Figure 1.

An ELISA-based approach for the large-scale profiling of Staphylococcus aureus adhesion to host ligands.A, schematic diagram of the high-throughput S. aureus adhesion assay. B, recognition of surface-adhered MRSA USA300 by the primary antibody is not dependent on the presence of cell wall-anchored proteins. A Nunc™ MaxiSorp™ microtiter plate was coated with MRSA USA300 JE2 and isogenic JE2 srtA::Tn (devoid of CWAs) propagated to an OD_600nm of 0.6. The cells were harvested, washed, and standardized to the various optical densities shown. Using the described ELISA, detection of surface adhered JE2 srtA::Tn was equivalent to that of the parental strain, indicating that recognition by the primary antibody is independent of CWA surface proteins. The values shown are the average mean ± S.D. of three duplicate biological replicates.