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Assessing the level of proteolytic activity in the adhesion attenuated mutants. Protease activity was assessed using TSA containing 1.5% Difco Skim Milk. The zones of clearance were measured using ImageJ. Each strain was measured with at least three biological replicates. Welch's t test (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001) was used to compare the mean length for each mutant to the mean of the parental strain (red dashed line).
