Table 1.
The S. aureus Genetic Adhesion Network Genes associated with S. aureus adhesion to a minimum of one of three host ligands (keratin, fibronectin, and fibrinogen)
Adhesion was assessed by titrating each host ligand (Fig. S1). For creation of the Genetic Adhesion Network, a final concentration was selected for each ligand, as indicated. The value listed for each mutant strain is the average percentage of adhesion relative to wild-type JE2 (three biological replicates), with lower values indicating a greater reduction in adhesion to a specific ligand. Genes mazE (SAUSA300_2026), recD2 (SAUSA300_1576), rpiR (SAUSA300_2264), and ydiL (SAUSA300_1984) are provisional names provided on the basis of their predicted structure and function. One gene, labeled hypo, is unannotated (SAUSA300_0602). p values were calculated using the two-tailed unpaired Student's t test; statistically significant (*, p ≤ 0.05) decreases in adhesion, compared with wild-type JE2, were used to create the Genetic Adhesion Network (Fig. 5).
| Gene | Locus | Keratin (0.125 μg/well) % adhesion | Keratin p value | Fibronectin (0.125 μg/well) % adhesion | Fibronectin p value | Fibrinogen (0.25 μg/well) % adhesion | Fibrinogen p value |
|---|---|---|---|---|---|---|---|
| agrC | SAUSA300_1991 | 106.8 | 0.57 | 102.4 | 0.75 | 77.0 | 0.040* |
| arlR | SAUSA300_1308 | 107.4 | 0.33 | 111.5 | 0.25 | 57.9 | 0.00096* |
| atl | SAUSA300_0955 | 51.6 | 0.026* | 23.7 | 0.0015* | 50.7 | 0.0044* |
| clfA | SAUSA300_0772 | 104.0 | 0.72 | 130.1 | 0.20 | 44.4 | 0.00050* |
| clfB | SAUSA300_2565 | 42.2 | 0.0055* | 81.4 | 0.45 | 101.9 | 0.26 |
| codY | SAUSA300_1148 | 68.3 | 0.038* | 49.4 | 0.012* | 97.2 | 0.80 |
| fmtA | SAUSA300_0959 | 60.5 | 0.028* | 73.2 | 0.12 | 76.7 | 0.049* |
| fmtC | SAUSA300_1255 | 96.1 | 0.44 | 82.7 | 0.13 | 54.5 | 0.00093* |
| hypo | SAUSA300_0602 | 100.4 | 0.95 | 100.4 | 0.97 | 65.1 | 0.026* |
| mazE | SAUSA300_2026 | 98.9 | 0.92 | 68.9 | 0.0058* | 96.3 | 0.75 |
| recD2 | SAUSA300_1576 | 41.8 | 0.00029* | 38.4 | 0.0019* | 20.6 | 0.000082* |
| rpiR | SAUSA300_2264 | 116.6 | 0.050 | 67.1 | 0.0056* | 136.9 | 0.020 |
| rpoF | SAUSA300_2022 | 18.6 | 0.0013* | 25.7 | 0.0012* | 35.2 | 0.0018* |
| rsbU | SAUSA300_2025 | 16.5 | 0.0012* | 17.0 | 0.00082* | 35.3 | 0.0016* |
| saeR | SAUSA300_0691 | 108.1 | 0.43 | 79.6 | 0.030* | 121.7 | 0.056 |
| saeS | SAUSA300_0690 | 104.9 | 0.44 | 86.0 | 0.037* | 117.5 | 0.016 |
| sarA | SAUSA300_0605 | 26.0 | 0.00016* | 29.6 | 0.000051* | 17.7 | 0.000085* |
| sarS | SAUSA300_0114 | 104.3 | 0.55 | 70.4 | 0.014* | 116.1 | 0.14 |
| srtA | SAUSA300_2467 | 20.9 | 0.0015* | 21.4 | 0.00083* | 34.4 | 0.0016* |
| ydiL | SAUSA300_1984 | 102.8 | 0.86 | 115.7 | 0.068 | 69.0 | 0.015* |