Table 2.
Primers used in this study
| Primers | Sequence (5′-3′) | Transposon primer used | Source |
|---|---|---|---|
| Confirmation of bursa aurealis transposon insertion site | |||
| Upstream | CTCGATTCTATTAACAAGGG | Bae et al. (2008) | |
| Buster | GCTTTTTCTAAATGTTTTTTAAGTAAATCAAGTAC | Bae et al. (2008) | |
| New_Upstream | CTTCAAACTTGACTTCAGC | This study | |
| New_Buster | CCAGTCTGGATCCAGTTG | This study | |
| accC_Tn_check | TTCGTTGTTTAATTGCG | Buster | This study |
| arlR_Tn_check | TTGATTACGGTGCAGA | Upstream | This study |
| atl_Tn_check | GTTGCATTAACGCTTGTAG | Upstream | This study |
| clfA_Tn_check | AACACGCAATTCGGAA | Buster | This study |
| clfB_Tn_check | TTGAAAAAAAGAATTGATTATTTGTC | Buster | This study |
| codY_Tn_check | ACGAGAGAGTTAAACACG | Upstream | This study |
| fmtA_Tn_check | GGTTGCGCCGTCTAAAC | fmtA_r versus_check | This study |
| fmtA_rversus_check | CACCCTTCGTATTGTAAGG | fmtA_Tn_check | This study |
| fmtC_Tn_check | GGCATCGCTTGTTATTC | Upstream | This study |
| fnbA_Tn_check | CTTAGGTACGGCATTAG | Buster | This study |
| fnbB_Tn_check | GCAATCTTAGATACGGC | Buster | This study |
| GNAT4_Tn_check | GAGACTTGTTTCGACAG | Upstream | This study |
| graR_Tn_check | TGGGTGATATGGATGC | Upstream | This study |
| hypo_rversus_check | GCAAGTGGCAACTCTATTG | New_Buster | This study |
| ilvE_new_check | GCAATCACCATGTCACAAG | ilvE_rversus_check | This study |
| ilvE_rversus_check | GTACAACGACTCTCCAAC | ilvE_new_check | This study |
| mazE_Tn_check | TGATTAGACGAGGAGATG | Upstream | This study |
| PutLipo_Tn_check | GACACTGGGATGTTTAC | Upstream | This study |
| RecD2_Tn_check | TGTCAGACCCTACACT | Upstream | This study |
| rot_Tn_check | GCATAAGTTAGCACATACAA | Buster | This study |
| rpiR_Tn_check | CGCGTTAAACAACAATAGC | New_Upstream | This study |
| rpoF_Tn_check | GCGAAAGAGTCGAAATC | Buster | This study |
| rsbU_Tn_check | GCCTGAAGACATTGTCG | Upstream | This study |
| rsbW_rversus_check | TTATCGAAATGCGCG | Buster | This study |
| sarA_Tn_check | GAGTTGTTATCAATGGTCAC | New_Upstream | This study |
| sarS_Tn_check | AGTGCATATACAAGGAGA | NaT_fwd_check | This study |
| sdcS_NaT_Tn_check | GTGCGGGACAACTTATTG | NaT_fwd_check | This study |
| NaT_fwd_check | GCACTGATTAAGTTTACCC | SdcS_NaT_Tn_check | This study |
| spa_rversus_check | CTAGGTGTAGGTATTGCATC | New_Buster | This study |
| srtA_Tn_check | TTATTTGACTTCTGTAGCTACAAAGATTTTACG | Upstream | This study |
| srtB_Tn_check | TTAACTTACCTTAATTATTTTTGCGAC | Upstream | This study |
| vraG_Tn_check | TGGCGTTAATTATGACC | Upstream | This study |
| xerC_Tn_check | GAATCATATTCAAGATGCGT | Upstream | This study |
| yabJ_rversus_check | CACAACAAGATTACCGG | Upstream | This study |
| YdiL_Tn_check | GGGCATCATTGCTAACTG | New_Buster | This study |
| YfeH_Tn_check | ATGTTGCGACATTAGG | Upstream | This study |