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. 2021 Jan 13;295(49):16888–16896. doi: 10.1074/jbc.AC120.014343

Figure 2.

Figure 2

F-actin–depolymerizing agents reduce the stiffness of Cltc KD mESCs.A and B, representative confocal micrographs showing actin filaments in shScrambled, shCltc1, and shCltc3 mESCs treated with LatA (0.1 μm) for 12 h (A) and under Pfn1 KD (Pfn1i) conditions and stained using phalloidin (B). Scale bar, 10 μm (n = 15). C–E, bar graph (C) and table (D) showing Young's modulus (E) of shScrambled-, shCltc1-, and shCltc3-treated mESCs under the mentioned conditions. Significance was calculated with respect to the corresponding untreated samples. *, p < 0.05; ***, p < 0.0001 by Student's t test. Error bars, S.E. over 17–20 cells with 100 measurements on each cell (n denotes the number of cells analyzed).