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. 2021 Jan 13;295(49):16888–16896. doi: 10.1074/jbc.AC120.014343

Figure 3.

Figure 3

CLTC is essential for restoration of pluripotency in the context of actin modulation in mESCs.A and B, RT-qPCR analysis of pluripotency markers (A) and differentiation markers (B) in shScrambled, shCltc1, and shCltc3 upon treatment with either DMSO, LatA (0.1 μm), or CytoD (0.2 μm) for 12 h. The bar graph shows the expression of markers in mESCs under the indicated conditions relative to the relevant shScrambled control. Control is shown as a dotted line at 1. For all experiments, error bars represent S.D. (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001 by Student's t test. C, graph showing the number of Oct4-GFP–positive colonies obtained at day 14 of reprogramming per 900 Oct4-GFP MEFs. Significance was determined by one-way analysis of variance followed by post hoc Tukey–Kramer test. a, p value <0.05 compared with OSK + miR-302b; b, p value <0.05 compared with OSK + miR-294.