Figure 1.

circUBAP2 expression is upregulated in OS and associated with poor prognosis. (A) circUBAP2 expression in OS tissues as determined via RT-qPCR. (B) Kaplan-Meier analysis demonstrated an association between circUBAP2 expression and overall survival in patients with OS. (C) Receiver operating characteristic curves indicated that circUBAP2 effectively distinguished OS tissues from paired non-cancerous tissues (AUC =0.7664; cut-off value =0.524). (D) circUBAP2 expression in OS cells as determined via RT-qPCR. RNase treatment in (E) HOS and (F) SaOS-2 cells significantly decreased UBAP2 expression, but did not significantly alter circUBAP2 expression. ^*P<0.05 and ^**P<0.01. circ, circular RNA; UBAP2, ubiquitin associated protein 2; OS, osteosarcoma; RT-qPCR, reverse transcription-quantitative PCR; AUC, area under the curve; CI, confidence interval.