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. 2021 Jan 27;58(3):298–311. doi: 10.3892/ijo.2021.5178

Figure 3.

Figure 3

circUBAP2 sponge miR-204-3p and miR-204-3p is downregulated in OS. (A) Bioinformatics analysis predicted binding sites between miR-204-3p and circUBAP2. Luciferase reporter assays were performed to verify the interaction between miR-204-3p and circUBAP2 in (B) HOS and (C) SaOS-2 cells. RNA immunoprecipitation assays were performed to further verify that circUBAP2 bound to miR-204-3p in (D) HOS and (E) SaOS-2 cells. (F) Effect of circUBAP2 knockdown on miR-204-3p expression in HOS and SaOS-2 cells as determined via RT-qPCR. (G) miR-204-3p expression in OS tissues as determined via RT-qPCR. (H) miR-204-3p expression was negatively correlated with circUBAP2 expression in OS tissues. (I) Kaplan-Meier analysis demonstrated an association between miR-204-3p expression and overall survival in patients with OS. (J) miR-204-3p expression in OS cells as determined via RT-qPCR. *P<0.05 and **P<0.01. circ, circular RNA; UBAP2, ubiquitin associated protein 2; miR, microRNA; OS, osteosarcoma; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; WT, wild-type; Mut, mutant; Ago2, argonaute RISC catalytic component 2; si, small interfering RNA.