(A) RT-LAMP-BART bioluminescent droplet images prepared using an optimized BART reagent mixture. The target RNA template concentration was 104 copies/mL. (A1) Droplet count of the reaction shown in (A). Positive droplets: 9; negative droplets: 19,574; total droplets: 19,583. (B) RT-LAMP-BART bioluminescent droplet images prepared using an optimized BART reagent mixture. The target RNA template concentration was 105 copies/mL. (B1) Droplet count of the reaction shown in (B). Positive droplets: 77; negative droplets: 17,309; total droplets: 17,386. (C) RT-LAMP-BART bioluminescent droplet images prepared using an optimized BART reagent mixture. The target RNA template concentration was 106 copies/mL. (C1) Droplet count of the reaction shown in (C). Positive droplets: 669; negative droplets: 18,428; total droplets: 19,097.