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. 2021 Jan 20;22(3):1017. doi: 10.3390/ijms22031017

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) RT-LAMP-BART bioluminescent well images obtained when utilizing an optimized BART reagent mixture. 1 and 2 meant the target RNA template concentration from 10⁴ to 10³ copies/mL. 3 meant the negative control sample. Positive wells from 1 to 3: 96, 60, 0. Negative wells from 1 to 3: 0, 30, 96. The total well wells from 1 to 3: 96, 96, 96. (B) The same samples in (A) were tested by qPCR. (C) The same samples in (A) were tested via a colorimetric kit, wherein blue and clear correspond to positive and negative, respectively. The negative control sample was derived from the SARS-CoV-2 Orf1ab Visual RT-LAMP Kit.