Figure 1.

AGCT cell line establishment and drug screen work flow. Tumor samples were processed within 4 h after collection. Confirmation of the FOXL2 c.402C > G mutational status of the tumor and cell line was performed by Sanger sequencing. Upon sufficient expansion, cells were seeded in a 384 well plate and drugs were applied 24 h after cell seeding. The response to treatment was analyzed 72 h after drug application by measuring cell viability.