Fig. 3.

Regulation of miR-30a by PPARγ. A: a proliferator-activated receptor-γ (PPARγ) binding site is in the promoter region of the miR-30a gene, as detected by 2 different antibodies (Ab1 and Ab2). The binding site is highly conserved among mammals (Mammal Cons). B: human adipocytes were treated with rosiglitazone for 4 days. Expression levels of miR-30a, miR-30b, miR-30c, miR-30d, miR-30e, fatty acid-binding protein 4 (FABP4), adiponectin (ADIPOQ), and uncoupling protein 1 (UCP1) were determined by quantitative PCR (qPCR) (n = 3 ± SE). C: mature adipocytes were transfected with PPARγ siRNA or scrambled control. qPCR was used to measure levels of PPARγ and miR-30a (n = 7 ± SE). Expression levels were normalized to TBP or RNU48. Statistical significance between DMSO and rosiglitazone for each molecule was calculated using unpaired, 2-tailed Student’s t test with Sidak-Bonferroni correction for multiple comparisons. *P < 0.05.