Fig. 1.

Gene and protein architecture of FAM134B variants. A: human and mouse FAM134B genome organization scheme based on the National Center for Biotechnology Information reference sequencing (RefSeq) and the exon annotation based on the University of California Santa Cruz genome browser. Note that isoforms differ from each other by transcription start sites. Dashed arrows faced inwards indicate the location of selected oligonucleotides for RT-PCR. *Exons deleted for Fam134b knockout mouse model. B: schematics of human FAM134B-1 and FAM134B-2 proteins. The latter is further detailed as NH₂-terminal amino acid sequence across species in the alignment box. V-shaped black rods represent hairpin structures of transmembrane regions. Red boxes/rods indicate the LIR motif, the target of GABARAP/LC3. Prefixes h-, b- and m- represent human, bovine, and mouse FAM134B sequences, respectively.