Fig. 2.
FAM134B isoforms are differentially expressed in human tissues. A: GTEx RNA-sequencing data of human FAM134B isoforms represented as Log2 [mean reads per kilobase million (RPKM) + 1] (SDs are not shown). B: FAM134B isoform expression levels in healthy human tissue panel demonstrated by the reverse transcriptase-PCR (RT-PCR). Equal loading control PCR performed by using GAPDH primers. The tissue name coding is as follows: Br, brain; Ts, testis; Sp, spleen; Ov, ovaries; Pr, prostate; Si, small intestine; Lv, liver; Co, colon; Pc, pancreas; Kd, kidney; Ht, heart; Sm, skeletal muscle; Ty, thymus. For each tissue, FAM134B-1 and FAM134B-2 bands were loaded at left- and right-hand side, respectively. Predicted band sizes according to in silico PCR is as follows: FAM134B-1, 450 bp; FAM134B-2, 449 bp.