Skip to main content
. 2020 Oct 14;319(6):G733–G747. doi: 10.1152/ajpgi.00094.2020

Fig. 5.

Fig. 5.

Starvation induces FAM134B-2 in liver, muscle, and heart in mice. A: protein levels of FAM134B-2, p62, and LC3B in livers of mutant and wild-type mice (12-wk-old, male) upon 48-h starvation. *FAM134B-2 is observed near 55 kDa, along with the additional band at 35 kDa. **Nonspecific antibody binding. B: mRNA levels of Fam134b isoforms in 48-h starved mouse liver. Numbers indicate the mouse Fam134b variants; H, mouse HPRT. Expected band sizes according to in silico PCR are as follows: isoform-1, 354 bp; isoform-2, 343 bp; isoform-3, 474 bp; isoform-4, 231 bp; isoform-5, 256 bp; isoform-6, 286 bp; isoform X1, 469 bp; isoform X2, 473 bp. C and D: tissue-specific changes in FAM134B-2 levels upon 36-h starvation (18-mo-old mice, male) are demonstrated for skeletal muscle (gastrocnemius) and heart (C) and for pancreas and stomach (D).