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. Author manuscript; available in PMC: 2021 Mar 6.
Published in final edited form as: Leukemia. 2020 Jun 27;35(3):901–905. doi: 10.1038/s41375-020-0942-6

Figure 1. Increased HSC survival in Gata2 −77+/− mice.

Figure 1.

Control (wild-type C57BL/6), Gata2 −77+/− (−77) and Gata2+/− (Gata2+/−) mice were sacrificed at 6-weeks of age. (A) Gating strategy for hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs). HSCs are defined as Lin− Sca1+ c-Kit+ CD41− CD48− CD150+ cells and MPPs are defined as Lin− Sca1+ c-Kit+ CD41− CD48− CD150− cells. HSCs and MPPs were quantified in bone marrow (BM) and spleen (SP). (B, C) Quantification of apoptotic HSCs (B) and MPPs (C) from BM using Annexin V and DAPI. (D, E) Cell cycle analysis of BM HSCs (D) and MPPs (E) using Ki67 and DAPI. The results are presented as means + SD. * P<0.05, ** P<0.01; *** P<0.001.