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. 2021 Jan 21;22(3):1046. doi: 10.3390/ijms22031046

Figure 4.

Figure 4

Upregulation of FAP enzymatic activity by human recombinant TGFbeta-1 protein in different cell types originating from human glioblastomas (GBMs). The cells were cultured in the absence or presence of recombinant TGFbeta-1 for 72 h and their lysates were assayed for FAP enzymatic activity and total protein concentration. (A) Upregulation of FAP enzymatic activity in TGFbeta-1-treated permanent glioma cell lines. (B) Upregulation of FAP enzymatic activity in TGFbeta-1-treated non-stem glioma cell cultures, but absence of or negligible increase in FAP enzymatic activity in TGFbeta-1-treated glioma stem-like cell cultures. (C) Upregulation of FAP enzymatic activity in TGFbeta-1-treated human brain vascular pericytes (HBVP) and glioblastoma-derived FAP+ mesenchymal (pFAP) cell cultures. (D) Upregulation of FAP enzymatic activity in TGFbeta-1-treated endothelial cell (pEC) cultures isolated from human GBMs, but not in human umbilical vein endothelial cells (HUVEC). Results are presented as mean ± SD from six parallel cell cultures measured in triplicate. In (A–D), * p < 0.05, one way ANOVA, Tukey’s post hoc test.