Table 2.
Antibodies used for the characterization of cell cultures derived from human glioblastomas.
| Antibody, Source | Dilution | Temperature, Incubation Time | Secondary Antibody (1:500, Room Temperature, 1 h) |
|---|---|---|---|
| Anti-FAP mouse IgG1 kappa isolated from F19 mouse hybridoma (ATCC CRL-2733) | 56 μg·mL−1 | 4 °C, overnight | Anti-mouse Alexa Fluor 488 (Invitrogen, A21202) |
| Anti-TE-7 (CBL271, Millipore) | 1:100 | room temperature, 1 h | Anti-mouse Alexa Fluor 488 (Invitrogen, A21202) |
| Anti-PDGFRbeta (LS-C11443, LSBio) | 1:50 | 4 °C, overnight | Anti-mouse Alexa Fluor 488 (Invitrogen, A21202) |
| Anti-vWF (A0083, Dako) | 1:200 | room temperature, 1 h | Anti-rabbit Alexa Fluor 488 (Invitrogen, A11010) |
| Anti-GFAP (11-255-M001, Exbio) | 1:200 | 4 °C, overnight | Anti-mouse Alexa Fluor 488 (Invitrogen, A21202) |
| Anti-beta III Tubulin (TU-20, ab7751, Abcam) | 1:250 | 4 °C, overnight | Anti-mouse Alexa Fluor 488 (Invitrogen, A21202) |