Table 3.

Primers and TaqMan probes for real-time RT-PCR quantification of expression of human FAP and ACTB mRNAs.

Human Transcript	GenBank Accession No.	Primer Names	Sequences of Primers and TaqMan Probes
Fibroblast activation protein (FAP)	NM_004460.4	Forward primer:	5′-TCTGCTGTGCTTGCCTTATTG-3′
		Reverse primer:	5′-ATGAAGATATTCTTGTCCTGAAATCC-3′
		TaqMan probe:	5′-(6-FAM)TGCATTGTCTTACGCCCTTCAAGAGTTCA(TAMRA)-3′
β-actin (ACTB)	NM_001101.2	Forward primer:	5′-ATGGCCACGGCTGCTT-3′
		Reverse primer:	5′-CCATGCCCAGGAAGGAA-3′
		TaqMan probe:	5′-(6-FAM)CCCTGGAGAAGAGCTACGAGCTGCCT(TAMRA)-3′