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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Curr Protoc Immunol. 2020 Dec;131(1):e116. doi: 10.1002/cpim.116

Figure 3. Visualization and quantification of a representative FRNT assay.

Figure 3.

A) Representative FRNT assay results show a comparison between the viral foci of a convalescent patient versus a healthy control at three different time points, 24, 48 and 72 hours along with a mock infection control. Antibody neutralization is quantified by counting the number of foci for each sample using the Viridot program (Katzelnick et al., 2018). B) The percentage neutralization of a convalescent patient is shown here. Each specimen is tested in two independent assays performed at different times. The FRNT50 titer is interpolated using a 4-parameter nonlinear regression in GraphPad Prism 8.4.3. C) The dot plot depicted here shows FRNT50 titers of 9 healthy control individuals versus 10 convalescent patients.