Table 1. Velocities and diffusion coefficients obtained from MD/MSD analysis.

Values are presented for experimental data (i) and simulated using the fully quantified model for the collective motion of Cin8 (ii). na, not analyzed due to the paucity of this size of clusters.

(i) Experimental	VMD (nm/s)*		VMSD (nm/s)*		D × 103 (nm2/s)†
Nucleotide‡	ATP		ATP		ATP		ADP
[KCl] (mM)	165	110	165	110	165	110	165
Single molecules	−237 ± 8 (71)	−18.1 ± 0.2 (39)	229 ± 66 (71)	31 ± 14 (39)	102 ± 11 (71)	22.3 ± 0.8 (39)	10.4 ± 0.1 (28)
Pairs of molecules	23.2 ± 0.7 (25)	−11.9 ± 0.1 (38)	21 ± 5 (25)	31 ± 6 (38)	5.0 ± 0.8 (25)	12.3 ± 0.2 (38)	7.0 ± 0.3 (21)
Higher oligomers	10.7 ± 0.2 (16)	1.12 ± 0.02 (9)	14 ± 2 (16)	26 ± 3 (9)	1.1 ± 0.1 (16)	1.6 ± 0.1 (9)	na
(ii) Simulation§	VMD (nm/s)		VMSD (nm/s)		D × 103 (nm2/s)
Single molecules	−229		227		109
Pairs of molecules	23.0		22.0		5.30
Higher oligomers║	16.8		15.7		2.26
Clusters with three molecules	17.8		16.5		2.38
Clusters with four molecules	11.5		9.58		1.79
Clusters with five molecules	9.86		8.25		1.32

*V_MD and V_MSD represent velocities evaluated from MD and MSD analyses, respectively. Negative values of velocity represent motility in the minus-end direction. Averages ± SEM are presented. Values in parentheses represent the numbers of Cin8 motors analyzed.

†Diffusion coefficient evaluated from the MSD analysis.

‡Nucleotide concentrations are 1 mM.

§Values were obtained by fitting the linear function 〈x(t)〉 = v_MDt to the temporal evolution of the MD, and the quadratic function $\langle \mathit{x}{(\mathit{t})}^2\rangle ={\mathit{v}}_{\text{MSD}}^2{\mathit{t}}^2+2{\mathit{D}}_{\text{MSD}}\mathit{t}$ to the temporal evolution of the MSD, where x(t) denotes the position of the respective particles at time t on the MT, and angle brackets refer to an ensemble average over different trajectories. An attachment rate of ${\mathit{k}}_0^{\text{on}}=2\bullet {10}^{-5} {\mathrm{s}}^{-1}$ was used. A lattice size of L = 5000 was used. Other model parameters are listed in table SB1. For further description on the evaluation and tracking method, see sections SB3 and SB4. Values correspond to experiments under high ionic strength conditions (165 mM KCl) in the presence of saturating ATP concentration.

║Parameters obtained from MD and MSD analyses of clusters containing three, four, and five Cin8 molecules, listed in the three lower rows of this table.