Fig. 5. RNA capture.

(A) Surface view of the RNA capture complex (nucleic acids as cartoon) with superimposed ρ₆ from the primed complex. Arrow, movement of ρ₆ during the transition from the primed to the RNA capture state. (B) Close-up views on ρ₆^PBS with bound RNA. Angled arrows, direction of intervening RNA region that might ascend 5’-to-3’ through the open ρ ring and return on the outside. Inset, details of RNA binding at ρ₆^PBS. 5’-portion of the RNA and selected ρ₆^PBS residues as sticks colored by atom type. In this and the following figures: Carbon RNA, red; carbon ρ residues, magenta; oxygen, light red, nitrogen blue; phosphorus, orange. (C) Quantification of β-gal activity derived from a reporter construct (scheme) in cells with ρ^WT or ρ^Y80c, in the presence of the indicated plasmid-encoded β’ variants. Values represent means ± SEM of at least nine independent experiments.