Table 1. SARS-CoV-2 specific primers used for partial sequences of particular region.
| (a) Primers used in Polymerase Chain Reaction | ||||
| Stage polarity | Primer name | Nucleotide position | Nucleotide sequence (5’-3’) | |
| hCoV-A set | PCR 1st Sense | SC2-A-1-1 | 8561–8581 | GTTAATAATTGGTTGAAGCAG |
| PCR 1st Antisense | SC2-A-1-2 | 8865–8884 | TATCGTGCCAGGCAAACCAG | |
| PCR 2nd Sense | SC2-A-2-1 | 8630–8650 | TTAATAACACCTGTTCATGTC | |
| PCR 2nd Antisense | SC2-A-2-2 | 8834–8854 | ACCCACTTCTCTTGTTATGAC | |
| hCoV-B set | PCR 1st Sense | SC2-B-1-1 | 10922–10943 | GAATTTACACCTTTTGATGTTG |
| PCR 1st Antisense | SC2-B-1-2 | 11280–11300 | GCTTAAAACCAGACAAACTAG | |
| PCR 2nd Sense | SC2-B-2-1 | 10957–10977 | AGGTGTTACTTTCCAAAGTGC | |
| PCR 2nd Antisense | SC2-B-2-2 | 11234–11254 | AATACGCATCACCCAACTAGC | |
| hCoV-C set | PCR 1st Sense | SC2-C-1-1 | 14199–14218 | AGAGTCACATGTTGACACTG |
| PCR 1st Antisense | SC2-C-1-2 | 14524–14545 | TAAAACTAAGTCTAGAGCTATG | |
| PCR 2nd Sense | SC2-C-2-1 | 14297–14318 | ATTTTAAATATTGGGATCAGAC | |
| PCR 2nd Antisense | SC2-C-2-2 | 14500–14521 | AGTTTACATCCTGATTATGTAC | |
| hCoV-D set | PCR 1st Sense | SC2-D-1-1 | 23249–23270 | CAACAATTTGGCAGAGACATTG |
| PCR 1st Antisense | SC2-D-1-2 | 23496–23516 | CTATTAAACAGCCTGCACGTG | |
| PCR 2nd Sense | SC2-D-2-1 | 23278–23297 | TACTGATGCTGTCCGTGATC | |
| PCR 2nd Antisense | SC2-D-2-2 | 23453–23472 | GAATAAACACGCCAAGTAGG | |
| hCoV-E set | PCR 1st Sense | SC2-E-1-1 | 25359–25380 | AAGGAGTCAAATTACATTACAC |
| PCR 1st Antisense | SC2-E-1-2 | 25658–25678 | GCAAAAGGTGTGAGTAAACTG | |
| PCR 2nd Sense | SC2-E-2-1 | 25394–25416 | TGGATTTGTTTATGAGAATCTTC | |
| PCR 2nd Antisense | SC2-E-2-2 | 25634–25654 | CAAACAACAACAGCAAGTTGC | |
| hCoV-F set | PCR 1st Sense | SC2-F-1-1 | 25878–25898 | TGAACATGACTACCAGATTGG |
| PCR 1st Antisense | SC2-F-1-2 | 26297–26317 | AGTACGCACACAATCGAAGCG | |
| PCR 1st and 2nd Sense | SC2-F-2-1 | 25919–25939 | AATCTGGAGTAAAAGACTGTG | |
| PCR 1st and 2nd Antisense | SC2-F-2-2 | 26259–26279 | GTAACTAGCAAGAATACCACG | |
| PCR 2nd Sense | SC2-F-3-1 | 25948–25968 | CACAGTTACTTCACTTCAGAC | |
| PCR 2nd Antisense | SC2-F-3-2 | 26216–26237 | CTATTAACTATTAACGTACCTG | |
| hCoV-G set | PCR 1st Sense | SC2-G-1-1 | 28612–28631 | ATGGGTTGCAACTGAGGGAG |
| PCR 1st Antisense | SC2-G-1-2 | 28903–28923 | GACATTTTGCTCTCAAGCTGG | |
| PCR 2nd Sense | SC2-G-2-1 | 28709–28729 | GGAACAACATTGCCAAAAGGC | |
| PCR 2nd Antisense | SC2-G-2-2 | 28874–28893 | TCAAGCAGCAGCAAAGCAAG | |
| hCoV- | PCR 1st Sense | ORF8-1-1 | 27645–27667 | TAAACTGTTCATCAGACAAGAGG |
| ORF8 set | PCR 1st Antisense | SC2-H-1-2 | 28314–28332 | GGTCCACCAAACGTAATGC |
| PCR 1st and 2nd Sense | ORF8-2-1 | 27706–27727 | GCGGCAATAGTGTTTATAACAC | |
| PCR 2nd Sense | ORF8-3-1 | 27779–27800 | CTTCTATTTGTGCTTTTTAGCC | |
| PCR 2nd Antisense | ORF8-2-2 | 28276–28297 | ATTTTGGGGTCCATTATCAGAC | |
| (b) Primers used in region-specific partial sequencing | ||||
| Primer name | Nucleotide position | Nucleotide sequence (5’-3’) | Target Nucleotide position | |
| hCoV-A set | SC2-A-2-1 | 8630–8650 | TTAATAACACCTGTTCATGTC | nt8782 |
| SC2-A-2-2 | 8834–8854 | ACCCACTTCTCTTGTTATGAC | ||
| hCoV-B set | SC2-B-2-1 | 10957–10977 | AGGTGTTACTTTCCAAAGTGC | nt11083 |
| SC2-B-2-2 | 11234–11254 | AATACGCATCACCCAACTAGC | ||
| hCoV-C set | SC2-C-2-1 | 14297–14318 | ATTTTAAATATTGGGATCAGAC | nt14408 |
| SC2-C-2-2 | 14500–14521 | AGTTTACATCCTGATTATGTAC | ||
| hCoV-D set | SC2-D-2-1 | 23278–23297 | TACTGATGCTGTCCGTGATC | nt23403 |
| SC2-D-2-2 | 23453–23472 | GAATAAACACGCCAAGTAGG | ||
| hCoV-E set | SC2-E-2-1 | 25394–25416 | TGGATTTGTTTATGAGAATCTTC | nt25563 |
| SC2-E-2-2 | 25634–25654 | CAAACAACAACAGCAAGTTGC | ||
| hCoV-F set | SC2-F-3-1 | 25948–25968 | CACAGTTACTTCACTTCAGAC | nt26144 |
| SC2-F-3-2 | 26216–26237 | CTATTAACTATTAACGTACCTG | ||
| hCoV-G set | SC2-G-2-1 | 28709–28729 | GGAACAACATTGCCAAAAGGC | nt28881 |
| SC2-G-2-2 | 28874–28893 | TCAAGCAGCAGCAAAGCAAG | ||
| hCoV-ORF8 set | ORF8-3-1 | 27779–27800 | CTTCTATTTGTGCTTTTTAGCC | nt27906-nt27907 |