(A) Wax-patterned paper sheet for the three-dimensional microPAD. (B) The 3D-microPAD with (a) being the paper working zones and (b) the paper auxiliary zones. (C) schematic of the screen-printed electrodes (c) represent the carbon working electrodes, (d) Ag/AgCl reference electrode, (e) carbon counter electrode, (f) silver conductive channel, and pad (g) transparent polyethylene terephthalate substrate. (D) Once stacking is complete, the paper working zones and paper auxiliary zones are aligned with the screen-printed working electrodes, count, and reference electrode.150 Reproduced with permission from Wang et al., Biosens. Bioelectron. 32(1), 238–243 (2012). Copyright 2012 Elsevier B.V.