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Early cell apoptosis detected by JC-1: (a) HLE-B3 cells cultured in a 96-well plate stained with JC-1; (b) fluorescence intensity quantification of aggregation (red channel); (c) fluorescence intensity quantification of monomer (green channel). Scale bars: 100 μm. Data are presented as the mean ± standard deviation from three independent experiments; ∗p < 0.05.
