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. 2020 Jul 7;140(1):7–19. doi: 10.1007/s00439-020-02202-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Breakdown of genetic tools used in published male infertility studies over the past decade. Based on an analysis of all published literature related to monogenic forms of male infertility, Sanger sequencing was the predominant tool used (82%) in 2010 but has since then seen a reduced usage down to 26% in 2019. In the same time period, NGS-based techniques gained ground to become currently the most common tool (61%)