Fig. 3.

Schematic representation of precise and efficient knock-ins by Combi-CRISPR. A dsDNA donor vector was used with Cas9 and two sgRNAs, one designed to cut the targeted genome sequences (sgRNA-2) and the other to cut both the flanked genomic region and one homology arm of the dsDNA plasmid (sgRNA-1 targeting). The NHEJ repair pathway dominantly induces indel mutations (purple) at the sgRNA-2 targeting site. Thereafter, the HDR pathway integrates a KI cassette (red) without any mutation at the sgRNA-1 targeting site. In some cases, the whole donor vector was integrated at the sgRNA-2 targeting site via NHEJ (black)