Fig. 5.

3D live cell two-photon imaging demonstrates glucose-dependent granule fusion is targeted towards ECM-coated coverslips. Islet cell clusters cultured on laminin-511-coated coverslips were stimulated with 15 mmol/l glucose and granule fusion events were recorded. (a) Representative images of exocytosis events (yellow) at the interface with the coverslip (0 μm) and at 10 μm above the coverslip. 3D models of all fusion events are also included. (b) Quantification of fusion events. B (bottom) represents the plane cells contact the coverslip (0 μm) and T (top) is the area of the cells above the coverslip (2–10 μm); **p=0.001. For control n=5 dispersed islet cell clusters obtained from 2 islet preparations, for Y15 treatment n=3 from 3 islet preparations. (c) Diagrammatic representation of the imaging method. Scale bars, 10 μm. Data represent mean ± SEM