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. 2020 Nov 20;22(1):77–91. doi: 10.1007/s10561-020-09867-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Extraction and identification of exosomes derived from human adipose-derived stem cells (hADSCs-Exos) during different time-spans of osteogenesis induction. The representative images of hADSCs-Exos during time-span of osteogenesis induction at Day 0 (Exo^0d, A), Day 1 to 14 (Exo^1−14d, B), and Day 15 to 28 (Exo^15−28d, C) were observed by transmission electron microscopy (TEM). Black arrows indicated the exosomes. Scale bar = 200 nm. D The particle size distribution of hADSCs-Exos was measured by dynamic light scattering (DLS) analysis, and the mean size of hADSCs-Exos was 125.8 ± 3.822 nm. E Western blot analysis showed that the specific surface markers of exosomes (CD9 and CD63) were detected