Fig. 6.
hADSCs-Exos promoted osteogenic differentiation of hADSCs in vitro. A Alkaline phosphatase (ALP) staining of hADSCs after incubation with exosomes (10, 15 and 20 μg/mL) from hADSCs after 14 days of osteogenic (Exo1−14d) on day 7 and day 14. Scale bar = 200 μm; B, C ALP activity normalized against the total protein level on day 7 and 14, respectively; D Western blot showed the expression of osteogenic-related proteins ALP and RUNX2 on day 14; E, F Relative protein level of ALP and RUNX2 on day 14, respectively. PM: proliferation medium; OM: osteogenic medium; *p < 0.05, **p < 0.01, and ***p < 0.001 compared with PM group, respectively. Δp < 0.05 and ΔΔp < 0.01 compared with OM group, respectively; ##p < 0.01 represents significant differences between compared groups