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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Cancer Immunol Res. 2020 Oct 6;9(2):227–238. doi: 10.1158/2326-6066.CIR-20-0396

Figure 2. Mice on a western diet have functionally impaired TILs independent of body mass.

Figure 2.

Mice on NC and WD were subcutaneously injected on each flank with 1×106 B16 tumor cells and treated with ICB on days 6, 10, and 14. On day 16, tumors were analyzed for (A) immune cell frequency by flow cytometry and graph shows data from 24 mice/treatment group (n=8/group for Tregs). (B) Representative flow plots show effector molecule expression on day 16. (C) The frequency of CD8+ TIL expressing GzmB was pooled from 4 independent experiments (15 mice/treatment group) using both male and female recipients. (D) The frequency of CD8+ TIL expressing IFNγ was pooled from 3 experiments (8–15 mice/treatment group). All error bars represent SEM and exact P values were calculated by two-way ANOVA for the bracketed groups. (E) Tumor volume and (F) CD8+ TIL granzyme B and IFNγ production were compared to the body mass of WD mice treated with ICB. Each square represents an individual mouse with inset lines representing the best fit for linear regression with the Pearson correlation coefficient (r) and exact P values indicated.