KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit-anti-RFP | Rockland | Cat# 600-401-379; RRID:AB_2209751 |
| Goat-anti-HB-EGF (anti-DTR) | R&D Systems | Cat# AF-259-NA, RRID:AB_354429 |
| Rabbit-anti-Fos | Synaptic Systems | Cat# 226 003, RRID:AB_2231974 |
| Sheep-anti-GFR alpha-like antibody | R&D Systems | Cat# AF5728, RRID:AB_2110592 |
| Anti-Digoxigenin-AP, Fab fragments | Roche | Cat# 11093274910, RRID:AB_514497 |
| Bacterial and Virus Strains | ||
| AAV9-CAG-Flex-tdTomato | Oh et al., 2014 | Addgene, Cat #51503-AAV9 |
| AAV9-Ef1a-DIO-hChR2(H134R)-mCherry | Gift from Karl Deisseroth | Addgene, Cat #20297-AAV9 |
| pAAV-hSyn-DIO-rM3D (Gαs)-mCherry | Gift from Bryan Roth | Addgene, Cat #50458 |
| pAAV-hSyn-DIO-hM3D(Gαq)-mCherry | Krashes et al., 2011 | Addgene, Cat #44361 |
| AAV9-Ef1a-DIO-EYFP | Gift from Karl Deisseroth | Addgene, Cat #27056-AAV9 |
| AAV1-CAG-Cre-mCherry | SignaGen | SignaGen, Cat #SL101117 |
| AAV1-CAG-mCherry | SignaGen | SignaGen, Cat #SL101127 |
| AAVrg-Flex-tdTomato | Gift from Edward Boyden | Addgene, Cat #28306-AAVrg |
| AAVrg-hSyn-DIO-GFP | Gift from Bryan Roth | Addgene, Cat #50457-AAVrg |
| Green Up cADDis cAMP sensor | Montana Molecular | Cat # U0200G |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Exendin-4 | Tocris | Cat #1933 |
| Clozapine N-oxide dihydrochloride | Tocris | Cat #6329 |
| Cisplatin | Tocris | Cat #2251 |
| Cinacalcet hydrochloride | Tocris | Cat #6170 |
| Forskolin | Tocris | Cat #1099 |
| Pramlintide | Tocris | Cat #5031 |
| Lipopolysaccharide, Salmonella typhimurium | Sigma-Aldrich | Cat #437650 |
| Diphtheria Toxin from Corynebacterium diphtheriae | Sigma-Aldrich | Cat #D0564 |
| IGEPAL-CA-630 | Sigma-Aldrich | Cat #I8896 |
| 6,7-dinitroquinoxaline-2,3-dione | Sigma-Aldrich | Cat #D0540 |
| DL-AP5 | Tocris | Cat #0105 |
| Tetrodotoxin | Tocris | Cat #1078 |
| FluoroGold | Santa Cruz Biotechnology | Cat #sc-358883 |
| Critical Commercial Assays | ||
| Papain dissociation system | Worthington Biochemical | Cat # LK003150 |
| TSA-Cy5.5 | Perkin-Elmer | Cat # NEL766001KT |
| MegaScript T7 kit | Invitrogen | Cat # AM1334 |
| Deposited Data | ||
| Raw images | This study | Mendeley data: doi:10.17632/tpw4c3g9m3.1 |
| Single-nucleus sequencing | This study | GEO accession number: GSE160938 |
| Single-nucleus sequencing analysis | This study | GitHub:http://github.com/jakaye/AP_scRNA |
| Experimental Models: Organisms/Strains | ||
| Glp1r-ires-Cre | Chang et al., 2015; Williams et al.,2016 | Deposited in Jackson laboratory (Cat#029282) |
| Slc17a6-ires-Cre (Vglut2) | Vong et al., 2011 | N/A |
| Calcr-ires-Cre | Pan et al., 2018 | N/A |
| Glp1r-KNOCKOUTCre | Sisley et al., 2014 | N/A |
| Glp1r-RESCUECre | Unpublished, gift from Randy Seeley (University of Michigan) | N/A |
| Calca-gfp | GENSAT | RRID:MMRRC_011187-UCD |
| Wild-type C57BL/6J | Jackson Laboratory | Cat #000664 |
| Rosa-LSL-L10GFP | Jackson Laboratory | Cat #024750 |
| Chat-gfp | Jackson Laboratory | Cat #007902 |
| Rosa-LSL-tdTomato | Jackson Laboratory | Cat #007908 |
| Gad2-ires-Cre | Jackson Laboratory | Cat #010802 |
| Rosa-LSL-DTR | Jackson Laboratory | Cat #007900 |
| Slc6a2-p2a-Cre | This study | N/A |
| Gfral-p2a-Cre | This study | N/A |
| Software and Algorithms | ||
| MATLAB R2019a | MathWorks | RRID: SCR_001622 https://www.mathworks.com/products/matlab.html |
| The R Project for Statistical Computing | R Core Team, 2019 | https://www.R-project.org/ |
| Seurat v3.1.5 | Butler et al., 2018; Satija et al., 2015 | https://satijalab.org/seurat/ |
| PRISM 7 | GraphPad | RRID: SCR_002798 https://www.graphpad.com/scientific-software/prism/ |
| FIJI | Schindelin et al., 2012 | RRID:SCR_002285 https://fiji.sc |
| BioRender | BioRender.com | RRID:SCR_018361 https://biorender.com |