Figure 2. VMC-derived apoE4 increases anxiety-like behavior, and impairs spatial learning and synaptic plasticity.

(A-C) Total distance travelled (A), time spent mobile (B), and center to total distance ratio (C) of iE3/Cre⁻ and iE3/Cre⁺ mice in the OFA tests. n.s., not significant, iE3/Cre⁻ vs. iE3/Cre⁺, Student t-test (A) and Mann-Whitney test (B and C). Data in this and subsequent figures represent mean ± SEM.

(D-F) Same analyses as in A, B and C except for comparing iE4/Cre⁻ and iE4/Cre⁺ mice. **P<0.01, iE4/Cre⁻ vs. iE4/Cre⁺, Student t-test (D). **P<0.01, *P<0.05, iE4/Cre⁻ vs. iE4/Cre⁺, Mann-Whitney test (E and F).

(G) Travelled distance to visible platform of iE3/Cre⁻ and iE3/Cre⁺ mice in the MWM test plotted against the training days. n.s., not significant, iE3/Cre⁻ vs. iE3/Cre⁺, repeated measures ANOVA.

(H) Left, normalized fEPSP responses to field stimulation in the CA1 region of hippocampal slices from iE3/Cre⁻ (n=5, all males) and iE3/Cre⁺ mice (n=6, all males). Right, averages of the last 5 min of fEPSP recording were quantified. n.s., not significant, iE3/Cre⁻ vs. iE3/Cre⁺, Mann-Whitney test.

(I and J) Same analyses as in G and H except for comparing iE4/Cre⁻ and iE4/Cre⁺ mice. *P<0.05, iE4/Cre⁻ vs. iE4/Cre⁺, repeated measures ANOVA (I). ***P<0.001, iE4/Cre⁻ vs. iE4/Cre⁺, Mann-Whitney test (J).

See also Figure S2 and Figure S5.