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. 2021 Feb 5;12:818. doi: 10.1038/s41467-021-21093-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The tree shows that the centipede β-PFTxs originated from at least two bacterial HGTs, one along the centipede or arthropod stem lineage (represented by the clade at the top of the tree with 94% bootstrap support), and one within the lithobiomorph lineage (represented by the clade of two lithobiomorph sequences lower down the tree). Centipede sequences are coloured blue (present in transcriptomes) and red (present in transcriptomes and venom proteomes). Highlighted sequences are Bacteria (pink), Euryarchaeota (brown), Protozoa (purple), Fungi (yellow), and Streptophyta (cyan). Metazoan sequences are not highlighted. Collapsed clades have the number of included sequences indicated in parentheses. For the uncollapsed tree see Supplementary Fig. 1. The tree was reconstructed using the WAG + R7 model and is displayed as midpoint rooted. Bootstrap support values are shown for each clade, and clades with support <50% are collapsed into polytomies. Clades without bootstrap values have >95% support. Non-centipede images are sourced from Phylopic (www.phylopic.org; credit for the Opiliones image is with Gareth Monger: https://creativecommons.org/licenses/by/3.0/).