Fig. 3. Colonic epithelial IL-33 expression is increased in Spry2^IEKO mice.

a Il33 expression was measured by qPCR in colonic homogenates from Spry2^FF and Spry2^IEKO mice (n = 17 FF, 13 KO; ***p = 0.0002). b IL-33 protein was measured in colonic epithelial scrapings by ELISA (n = 5 FF, 4 KO; *p = 0.0172). c Secreted IL-33 was measured in conditioned media from epithelial peels after 6 h incubation by ELISA (n = 3 per group; *p = 0.0314). d Expression of Spry2 (n = 11 FF, 9 KO cultures; ****p < 0.0001), Il33 (n = 11 cultures; *p = 0.0221), Dclk1 (n = 8 cultures), and Muc2 (n = 7 cultures) was assayed by qPCR in passaged colonoids generated from Spry2^FF and Spry2^IEKO mice. Il33, Dclk1, and Muc2 expression in Spry2^IEKO is presented relative to Spry2^FF mice (indicated by red dashed line n = 11 cultures). In figure, each colonoid culture was generated from a separate mouse and data are presented as mean ± SEM. Analyzed by two-sided t test.