Fig. 6. The p23 tail is required for GR folding in vivo.
Wild-type or sba1Δ yeasts cells were examined concerning their ability to mature heterologously expressed glucocorticoid receptor (GR). Cells either harbored the empty vector (e.v.) plasmid as control or expressed the indicated p23 mutants. The relative GR activities compared to the wild-type control are shown. Bars represent means ± SD of at least four independent experiments. Significance was evaluated by two-tailed t-testing (n.s. P ≥ 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001) and Welch correction was used if variances were significantly different between samples. [p(p23Δ17) = 0.06, p(p23Δ40) = 0.00014, p(p23Δ69) = 0.00012, p(p23Δ88) = 0.00054, p(p23Δ94) = 9.6 × 10−9, p(p23Δ178-188) = 0.00126, p(p23F121A) = 5.0 × 10−9, p(p23W124A) = 0.004, p(p23F121A/W124A) = 6.2 × 10−9].