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. 2021 Feb 5;11:3243. doi: 10.1038/s41598-021-82450-7

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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UPLC/ESI–MS/MS-based hormone profiling in flood stressed and control alfalfa genotypes. (A) The abundance of total abscisic acid, ABA, metabolites (B) abscisic acid glucose ester, ABAGE, (C) phaseic acid, PA, (D) ABA, (E) indole acetic acid-aspartic acid, IAA-Asp, (F) total auxin, (G) gibberellic acid GA19, (H) total gibberellic acid GA, and (I) total cytokinin in ng per gram leaf dry weight. Total ABA metabolites in ‘A’ comprises ABA, Dihydrophaseic acid (DPA), ABAGE (Abscisic acid glucose ester), Phaseic acid (PA), 7′-Hydroxy-abscisic acid (7′OH-ABA), neo-Phaseic acid (neo-PA), and trans-Abscisic acid (t-ABA). Total auxin in ‘F’ comprises Indole-3-acetic acid (IAA) and N-(Indole-3-yl-acetyl)-aspartic acid (IAA-Asp). Total gibberellic acid in ‘H’ comprises gibberellin 8, 9, 19, 20, 24, 29, 34, 44, 51, and 53. Total cytokinin in ‘I’ comprises (trans) Zeatin-O-glucoside (t-ZOG), (cis) Zeatin-O-glucoside (c-ZOG), (cis) Zeatin riboside (c-ZR), Dihydrozeatin riboside (dhZR), Isopentenyladenosine (iPR), Values are sample means ± SE, n = 3 individual plants as biological replicates. A significant difference from ANOVA was followed by Post hoc Tukey multiple comparisons test and indicated with different letters.