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. 2021 Feb 5;11:3243. doi: 10.1038/s41598-021-82450-7

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differentially expressed genes and their associated function upon flood stress in alfalfa. (A) Differentially expressed genes, DEG, in each genotype comparing flooding and well-drained control alfalfa plants, (B) Venn diagram illustrating commonality and uniqueness in DEG obtained by comparing flood-stressed WT to AC-Caribou, AAC-Trueman, SPL13RNAi-6 and miR156-A8 genotypes independently upon flooding, (C) Venn diagram illustrating commonly shared and unique DEG obtained by comparing flood-stressed and their respective well-drained control counterparts plants of each genotypes, (D) functional distribution of DEG generated by comparing flood-stressed WT to AC-Caribou, AAC-Trueman, SPL13RNAi-6 and miR156-A8 genotypes. Upper and lower panel numbers in ‘B’ and ‘C’ indicate increased and decreased DEG, respectively, compared to WT flood stress or the same genotype under control conditions respectively. Novaseq 6000-based RNAseq analysis was performed for three biological replicates for each treatment condition and genotype. Venn diagram was constructed using an online tool http://www.interactivenn.net/ from DEG.