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. 2021 Feb 5;12:819. doi: 10.1038/s41467-020-20806-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic representation of the architecture of DISC. b Western blot analysis of Glycerol Density Gradient purification of soluble Flag-FADD:Caspase-8 complexes. Western blots are representative of 3 independent experiments. c Negative-stain EM of FADD:Caspase-8 filaments purified from different gradient fractions (Fractions 10, 13 and 15). Micrographs are representative of 3 independent experiments. d Label-free quantitative mass spectrometry determination of FADD:Caspase-8 ratio in Glycerol Density Gradient fractions (Graph shows Mean ± SD, n = 3 technical repeats; from a representative experiment of 3 biologically independent experiments). e Immuno-gold labeling of FLAG-tag and Caspase-8 p18 subunit in purified filament complexes from Fraction 10 within the peak visualized by EM. Micrographs are representative of 3 independent experiments. f Structural model of FADD:Caspase-8 tDED single-chain complex. Source data are provided as a Source Data file.