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. 2021 Feb 5;12:819. doi: 10.1038/s41467-020-20806-9

Fig. 5. c-FLIP short (c-FLIPS) prevents Caspase-8 filament elongation altering the architecture of the complex.

Fig. 5

a Western blot of Glycerol Density Gradient profiles of FADD:full-length Caspase-8:FLAG-c-FLIPS complexes. Western blots are representative of 3 independent experiments. b Representative negative stain micrograph of FADD:full-length Caspase-8:FLAG-c-FLIPS complexes (Scale bar, 100 nm; inset, 25 nm). Micrographs are representative of 3 independent experiments. c Immuno-gold labeling of p18 subunit of Caspase-8 and FLAG-tag on purified FADD:full-length Caspase-8:FLAG-c-FLIPS complexes (Fraction 6), visualized by EM. Micrographs are representative of 3 independent experiments. d Reference-free 2D class averages generated in CryoSPARC of negative-stain EM of FADD:full-length Caspase-8:FLAG-c-FLIPS complexes. e Different orientations of low-resolution EM volume of FADD:full-length Caspase-8:FLAG-c-FLIPS complexes; side-view (left) and end-view (right). f Overlay comparison of FADD:full-length Caspase-8:FLAG-c-FLIPS complex negative-stain EM volume and FADD:full-length Caspase-8 complex EM volume; additional EM volume only present in c-FLIPS-inhibited complex is shown in yellow. Source data are provided as a Source Data file.