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. 2021 Feb 5;4:163. doi: 10.1038/s42003-021-01686-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Categorisation of lipids according to where they are found; b flow chart of traffic analysis showing the gross structure of the analysis. A, B and U are categories representing variables that appear in all compartments, in pairs of adjacent compartments and in only one compartment, respectively. Subscripts to these categories are pairs of one-letter codes indicating the direction of the traffic (reading left to right). Red connections show B-type lipid connections. Black connections show A-type lipid connections. The two strands of the flow chart represent separate analyses that use the same R code (see SI). Equations for the quantitative analysis are shown in Eqs (1) and (2).