Table 2.

Sequences of circular DNA, primers, and probes used in this study.

Type	Name	Sequence (5′ → 3′)	Length (bases)
Universal capture probe	CP	CGCAACTGAACTACTTGTCG–biotin	20
Blocking probe	BP	Biotin-TTTTTTTTTT	10
For N gene
Forward primer	Ng_SARS2_F	TCATCACGTAGTCGCAACAG	20
Reverse primer	Ng_SARS2_R	CAAAGCAAGAGCAGCATCAC	20
Circular DNA	RCA-Ng	TACGTGATGACGCAACTGAACTACTTGTCGCTGTAGTTCAAGATATCGCGTCCTACCTGTTGCGAC	66
Reporter probe	Ng-RP	CAAGATATCGCGTCCTAC–biotin	18
Linear target	Ng-LT	GTTCCTCATCACGTAGTCGCAACA GTTCAA	30
Mismatched target	Ng-MM	GTTCCTCATCACGTTCTCGCAACA GTTCAA	30
For S gene
Forward primer	Sg_SARS2_F	TACCCATTGGTGCAGGTATATG	22
Reverse primer	Sg_SARS2_R	AGTGTAGGCAATGATGGATTGA	22
Circular DNA	RCA-Sg	ACCAATGGGTCGCAACTGAACTACTTGTCGCTGTAGTTATTCTGTCATGCGCTCACATATACCTGC	66
Reporter probe	Sg-RP	ATTCTGTCATGCGCTCAC–biotin	18
Linear target	Sg-LT	GACATACCCATTGGTGCAGGTATATGCGCT	30
Mismatched target	Sg-MM	GACATACCCATTGGACCAGGTATATGCGCT	30