Table 3.
Cryo-EM data collection, refinement and validation statistics.
| Pangolin-CoV (EMD-12130) (PDB 7BBH) | |
|---|---|
| Data collection and processing | |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 51.8 |
| Defocus range (μm) | −1.5 to −3.0 |
| Pixel size (Å) | 1.08 |
| Symmetry imposed | C3 |
| Final particle images (no.) | 93 k |
| Map resolution (Å) | 2.9 |
| FSC threshold = 0.143 | |
| Map resolution range (Å) | 2.8–3.6 |
| Refinement | |
| Initial model used (PDB code) | 6ZGE |
| Model resolution (Å) | 3.0 |
| FSC threshold = 0.5 | |
| Map sharpening B factor (Å2) | −86.5 |
| Model composition | |
| Non-hydrogen atoms | 25,827 |
| Protein residues | 3189 |
| Ligands | 69 |
| B factors (Å2) | |
| Protein | 38.0 |
| Ligand | 70.9 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 0.707 |
| Validation | |
| MolProbity score | 1.36 |
| Clashscore | 3.02 |
| Poor rotamers (%) | 0.86 |
| Ramachandran plot | |
| Favored (%) | 96.13 |
| Allowed (%) | 3.87 |
| Disallowed (%) | 0.00 |