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. 2021 Feb 5;12:805. doi: 10.1038/s41467-021-21133-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–c Recipients were given 1-DEX, 4-DEX, or saline treatment as described in Fig. 1. Cytokine profiles of T cells from spleen and MLN were measured on day 25. Means ± SEM of % and yield of IL17A⁺IL-22⁻, IL17A⁺IL-22⁺, and IL17A⁻IL-22⁺ among CD4⁺ and CD8⁺ T subsets are shown in a–c, respectively. Each dot represents one mouse. n = 4 (1-DEX), 6 (Saline), 7 (4-DEX) combined from two replicates. d Representative flow cytometry patterns and means ± SEM of AHR⁺RORγt⁻ and AHR⁻RORγt⁺ among IL-17A⁺IL-22⁻ or IL-17A⁻IL-22⁺ CD4⁺ and CD8⁺ subsets. Each dot represents one mouse, n = 4, two replicates. e Means ± SEM of % and yield of IFN-γ⁺CD4⁺ and IFN-γ⁺CD8⁺ T in ileum. Each dot represents one mouse, n = 4, two replicates. f Recipients engrafted with splenic T from WT or IL-22^−/− donors combined with TCD-BM from WT donors were given 4-DEX treatment. The recipients of WT-T were given additional treatment of anti-IL-22 mAb or IgG at 150 µg every 3 days from days 12 to 21 after HCT. On day 25, histopathology of colon was evaluated. Representative micrographs (200×) and %mice with indicated histopathological scores are shown; n = 4 (WT-IgG & IL-22^−/− T), 12 (WT-anti-IL-22) biologically independent mice combined from two replicates. g, h Recipients engrafted with T cells from WT or T-RORγt^−/− combined with TCD-BM from WT donors were evaluated for cytokine profile of MLN T cells and histopathology of colon on day 25. g Means ± SEM of % and yields of IL17A⁻IL-22⁺ cells among CD4⁺ and CD8⁺ T-cell subsets; each dot represents one mouse, n = 5 (RORγt^−/− T), 7 (WT T) combined from two replicates. h Representative micrographs of colon (200×) and %mice with indicated histopathological scores; n = 8 biologically independent mice combined from two replicates. P value was determined by two-way ANOVA with Tukey’s (a–c) or Sidak’s (d), unpaired two-tailed Student’ t test (e, g). a ****p < 0.0001; yield of IL17A⁺IL-22⁻CD4⁺: **p = 0.0013, ***p = 0.0004; yield of IL17A⁺IL-22⁻CD8⁺: *p = 0^.0416^, **p = 0.0024 (spleen, saline vs 4-DEX),**p = 0.0074 (MLN, Saline vs 4-DEX); c ****p < 0.0001; yield of IL17A⁻IL-22⁺CD4⁺: **p = 0.0024 (saline vs 4-DEX) **p = 0.0050 (1-DEX vs 4-DEX); %IL17A⁻IL-22⁺CD8⁺: *p = 0.0256 (saline vs 1-DEX), *p = 0.0362 (1-DEX vs 4-DEX); ***p = 0.001,****p < 0.0001. Yield of IL17A⁻IL-22⁺CD8⁺: ****p < 0.0001, **p = 0.0040, *p = 0^.0493. d ****p < 0.0001. e *p = 0.0155, **p = 0.0032. g *p = 0.0410, ***p = 0.0003, ****p < 0.0001. Dexamethasone (DEX), MLN (mesenteric lymph node).