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. 2021 Feb 5;12:805. doi: 10.1038/s41467-021-21133-3

Fig. 3. IL-22 from Th/Tc22 cells causes dysbiosis and bacteria translocation.

Fig. 3

Lethally irradiated WT BALB/c recipients were engrafted with WT-TCD-BM alone or combined with  splenic T cells from WT or IL-22−/− C57BL/6 donors, and the recipients of splenic T cells were given 1-DEX or 4-DEX treatment as described in Fig. 1. Microbiome profile in feces from the ileum of recipients was measured on day 25 after HCT. a Diversity of ileal flora was determined by the number of species, Shannon index, and Fisher Index. Means ± SEM are shown. b Principal coordinate analysis of the ileal flora. c Bacterial composition at the species level of the ileal flora is depicted with individual mice displayed in each bar. d %Abundance of E. coli, Enterococcus_sp._FDAARGOS_553 and Lactobacillus murinus in the ileal flora. Means ± SEM are shown. e Bacteria colony numbers in the liver cell suspension culture plates, with one plate per recipient. Means ± SEM are shown. All data combined from two replicate experiments, each dot represents one mouse, n = 9 (TCD-BM), n = 6 (WT T-1-DEX & WT T-4-DEX & IL-22−/− T-4-DEX). Ordinary one-way ANOVA with Tukey’s correction for multiple comparisons was used in a, e. Kruskal–Wallis test with Dunn’s correction for multiple comparisons were performed with two-tailed p value in d. a Observed: **p = 0.0022 (WT-1-DEX vs. WT-4-DEX), **p = 0.0078 (WT-4-DEX vs. IL-22−/−T-4-DEX); Shannon Index: *p = 0.0351; Fisher Index: **p = 0.0025 (WT-1-DEX vs. WT-4-DEX), **p = 0.0077 (WT-4-DEX vs. IL-22−/−T-4-DEX). d, **p = 0.0016 (1-DEX vs 4-DEX), **p = 0.0042 (WT-4-DEX vs. IL-22−/−T-4-DEX); e ****p < 0.0001. T-cell-depleted bone marrow (TCD-BM), dexamethasone (DEX).