Fig. 7. Dysbiosis is required for Gut-aGVHD induced by IFNγ^−/−CD8⁺ T-derived Tc22 cells.

Gut-aGVHD was induced in BALB/c recipients with grafts from WT or IFNγ^−/− C57BL/6 donors as described in Fig. 4a. a, b Starting on the day of HCT, recipients of IFNγ^−/−CD8⁺T cells were separately housed or co-housed with recipients of WT CD8⁺ T cells. a Mean ± SEM of %Original bodyweight at each time point and recessive curves of %mice without diarrhea and %survival among total mice are shown. n = 12 combined from two replicate experiments. b %Abundance of Escherichia/Shigella, Clostridiaceae, and Lactobacillus from ileal fecal samples. Means ± SEM, n = 6 (co-house IFNγ^−/− CD8⁺ T), 8 (Non-co-house IFNγ^−/− CD8⁺ T & WT CD8⁺ T) from two replicate experiments. c Recipients of IFNγ^−/−CD8⁺ T cells were gavaged with a mixture of Ampicillin(1 g/L), Neomycin(1 g/L), Metronidazole(1 g/L), and Vancomycin (0.5 g/L) (4ABX) or PBS (250 µl/mouse/day) from days 0 to 7 following HCT.) Mean ± SEM of %original bodyweight at each time point and recessive curves of %mice without diarrhea and %survival among total mice are shown. n = 10 from two replicate experiments. Each dot represents one mouse, P value was determined by nonlinear regression (curve fit) (a, c bodyweight and diarrhea) with two-tailed p value, Log-rank test (a, c survival) with two-tailed p value, Kruskal–Wallis test with Dunn’s correction for multiple comparisons with two-tailed p value b. a ****p < 0.0001; b *p = 0.0463, ***p = 0.0002; c *p = 0.0480, ***p = 0.0002.