FIGURE 4.

The eIFiso4E protein levels are not increased in the eif4e1^KO mutant. (a) Western blot analysis on total protein extracts from mock‐inoculated wild‐type and TuMV‐GFP UK1‐inoculated plants at 21 days postinoculation (dpi). eIF4E1 and eIFiso4E protein accumulation was analysed by western blot on total plant protein extracts using specific antibodies. Equal loading was checked by western blot using anti‐actin antibodies. (b) Western blot analysis on m⁷GTP‐purified protein extracts from mock‐ or TuMV‐GFP UK1‐inoculated wild‐type and eif4e1^KO plants at 21 dpi. Total protein extracts (INPUT) were pulled down with γ‐aminophenyl‐m⁷GTP (C₁₀‐spacer)‐agarose beads (OUTPUT). After purification, the output fraction was analysed by western blot using anti‐eIFiso4E antibody while equal loading control was checked on total protein extracts (INPUT) by western blot using anti‐actin antibodies. Each lane represents immunoblotted protein extracts obtained from a single independent plant